팝업레이어 알림
COLLECTION, TRANSPORT & PRESERVATION SYSTEM
Since its establishment in 2008, WIZCHEM CO., LTD. has been researching, developing, and producing products with global competitiveness to meet customer needs in various fields such as functional chemical materials, biomedical cosmetics, hydrogel wound dressing and medical care. Based on these accumulated technology, for testing various viruses such as coronavirus, we have recently developed and produced in vitro diagnostic products such as viral transport medium(VTM) and swabs that collect and transport infectious virus samples, also viral DNA/RNA extraction kit and saliva collection kit for rapid and accurate diagnosis of diseases.
WIZCHEM’s Clinical Viral Transport Medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, including diagnostic tests, molecular biology techniques and conventional cell culture methods. We are following the WHO and CDC recommendations with swab.
WIZCHEM Viral Transport Medium is intended for the collection and transport of clinical specimens containing Virus, Chlamydia, Mycoplasma and Ureaplasma from the collection site.
The test procedures for quality control are based upon the quality control methods described in CLSI M40-A2 and others. VTM transport Medium is stable for 12 months at 5~25°C. Specimens in VTM medium are stable for 48 hours at room temperature.
This VTM collection and transport system is certified as Biopsy Procedure Kit, class l with product-license number by the Korean FDA.
Transport medium for sample collection and nucleic acid preservation for molecular diagnosis
-. All viruses(COVID-19, Respiratory Syncytial Virus, Influenza A and B, Herpes Simplex I and II, Cytomegalovirus, Echovirus, etc.)
-. Chlamydia tachomatis and pneumoniae
-. Mycoplasma hominis and pneumonia
-. Ureaplasma urealyticum
-. Enzyme Immuno Assays (EIA)
1) VTM medium transport specimens in safety by using self-standing conical Tube.
2) There are two types of swab applicators for the sample collection.
3) Specimens in VTM are stable for 48 hours at room temperature.
4) These products are medical devices certified as class I by the Korean FDA.
|
Component |
Volume |
Cat. No. |
Product Image(Swab Applicator) |
|---|---|---|---|
|
12ml Screw Cap Tube Nucleic acid Preservation Medium Nasopharyngeal Swab Applicator (NSA-T1) |
1ml 2ml 3ml |
VTM 1M-NSA-T1 VTM 2M-NSA-T1 VTM 3M-NSA-T1 |
|
|
12ml Screw Cap Tube Nucleic acid Preservation Medium Oropharyngeal Swab Applicator (OSA-T1) |
1ml 2ml 3ml |
VTM 1M-OSA-T1 VTM 2M-OSA-T1 VTM 3M-OSA-T1 |
|
|
12ml Screw Cap Tube Nucleic acid Preservation Medium Oropharyngeal Swab Applicator (OSA-T2) |
1ml 2ml 3ml |
VTM 1M-OSA-T2 VTM 2M-OSA-T2 VTM 3M-OSA-T2 |
|
|
12ml Screw Cap Tube Nucleic acid Preservation Medium Cervical Swab Applicator (CSA-T1) |
2ml 3ml |
VTM 2M-CSA-T1 VTM 3M-CSA-T1 |
|
Information and intended Use
1) Nasopharyngeal(NP) & Oropharyngeal(OP) Swab samples are the preferred specimens for respiratory virus rapid antigen and Real-Time PCR testing.
2) The NP & OP swab have a white plastic shaft, with 3 (NP) and 2(OP) different thicknesses respectively, ending in a flock tip.
3) There is a deep score mark(V) on the thick part as a breakpoint where it can be snapped into the transport medium.
|
Description |
Flocked Mini Tip Swab Applicator [NP & OP Flocked Swab] |
|---|---|
| The Swab Type | NSA-T1 & OSA-T1 |
| Full length of applicator | NP swab : 150mm & OP Swab : 155mm |
| Breakpoint Distance | NP swab 80mm, OP swab 30mm |
| Use | VTM, POCT diagnosis |
The competitiveness of our products compared to other products
The head of a typical nasal swab is short. Thus, a small amount of samples are often taken, and it causes pain when put into the nose by having a pointy tip.
However, WIZCHEM’s nasal swabs with long heads can take a lot of samples. And a round ball is added to the top of the fifth nasal swab to prevent pain during the test.
[Fig. 1] Remove the cover sheet of the Nuclear acid Preservation & Transport Medium.
[Fig. 2] Remove the protective tube of the sample collection swab and use the swab to collect the patient's samples.
[Fig. 3] Close the collected swab to the transport medium as shown in Figure 3, then apply downward force to cut.
[Fig. 4] Close the tube cap of the transport medium tightly, fill in the patient's information, and transport it to the examination institution.
Upper Respiratory Tract
Nasopharyngeal (NP) Swab + Oropharyngeal (OP) Swab
Nasopharyngeal (NP) Swab can be collected scratching the nasopharynx through the nostril.
Oropharyngeal (OP) Swab can be collected scratching the pharynx having your tongue down.
|
Symbol |
Explanation |
|---|---|
|
Catalog number |
|
CE mark |
|
In-vitro diagnostics medical device |
|
Batch code |
|
Expiration date |
|
Sufficient for <n> tests |
|
Consult instructions for use |
|
Temperature limit |
|
Do not re-use |
|
Do not re-sterilize |
|
Do not use if package is damaged. |
|
Caution, consult accompanying documents |
|
Manufacturer |
|
Manufacture date |
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
CDC is now recommending collecting only the NP swab, although OP swabs remain an acceptable specimen type. If both NP and OP swabs are collected, they should be combined in a single tube to maximize test sensitivity and limit use of testing resources. Please refer to the protocol in the "Microbiology Specimen Collection Manual".
COVICHEK™ Saliva Collection Kit is an easy to use DNA and RNA saliva collection device that stabilizes DNA and RNA long term in saliva sample.
The saliva collection tube clearly indicates the volume of saliva required after which the funnel is removed and cap replaced.
The device is fully specified for sample transport and storage.
1. COVICHEK™ Saliva Collection Kit is an easy to use DNA and RNA saliva collection device that stabilizes DNA / RNA long term in saliva sample.
2. The saliva collection tube clearly indicates the volume of saliva required after which the funnel is removed and cap replaced.
3. The collector is pre-filled and has a simple screw-on funnel for simple saliva delivery directly into the non-toxic stabilization buffer. The device is fully specified for sample transport and storage.
4. The unique funnel design prevents flow back and can also be used with our assisted option using foam swabs to collect saliva from babies, elderly or any donor unable to use the funnel.
- SCK–1M-FT-1 1ml Saliva Collector (SCK-1M) & Funnel Type 1 (FT-1)
- SCK–2M-FT-1 2ml Saliva Collector (SCK-1M) & Funnel Type 1 (FT-1)
- SCK–3M-FT-1 3ml Saliva Collector (SCK-1M) & Funnel Type 1 (FT-1)
1) Saliva has been studied thoroughly as a potential diagnostic tool and it is expected to become a substitute for other biological fluids such as serum or urine in disease diagnosis.
2) Saliva has been described as a good candidate for diagnosis of COVID-19, showing sensitivity and specificity comparable to nasopharyngeal swabs.
3) Saliva contains hormones, antibodies, growth factors, enzymes, microbes. Saliva is used for the diagnosis of several diseases including hereditary diseases, autoimmune diseases, malignancies, infections, dental caries and periodontal disease.
4) Additionally, saliva can be used for diagnosing oral diseases with relevance for systemic diseases or for monitoring of levels of hormones, drugs and bone turnover markers.
Transport medium for saliva sample collection and nucleic acid preservation for molecular diagnosis for All viruses(COVID-19, Respiratory Syncytial Virus, Influenza A and B, etc.) and for Enzyme Immuno Assays (EIA)
1) WIZCHEM Saliva Nucleic Acid Preservation & Transport(NAPT) Medium is a transport medium for preserving nucleic acids that can be stored reliably at room temperature after Saliva sampling - clinical specimens.
2) The sample can be safely transported through the use of a sample transport container, and the structure is convenient and centrifugal separation can be used using a self-standing mechanical tube.
3) Place the samples in a nucleic acid preservation medium and keep the DNA stable at room temperature for more than five months and RNA stable for more than a week.
4) Saliva Nucleic Acid Preservation & Transport(NAPT) Medium is stable for 12 months at room temperature.
5) Specimens in Saliva Nucleic Acid Preservation & Transport(NAPT) medium are stable for 48 hours at room temperature.
6) Saliva biomarkers have a potential to be an important guide in COVID-19 prognosis, making possible the development of point-of-care devices.
7) The test procedures for quality control are based upon the quality control methods described in CLSI M40-A2 and others.
1) This Saliva Viral DNA/RNA Collection Kit is suitable for rapid extraction of high purity viral nucleic acid from saliva, sputum, bronchus/alveolar lavage fluid as well as nasopharyngeal(NP) & oropharyngeal (OP) swabs.
2) The obtained nucleic acids can be directly used in downstream related experiments such as reverse transcription, PCR, RT-PCR, fluorescence quantitative PCR, second-generation sequencing and Northern hybridization.
1) Remove the cap and fit the funnel into the tube.
2) Add the amount of saliva needed for the funnel.
3) Remove the funnel and fit the cap.
4) Shake the tube from side to side.
1) Saliva collection is easier to perform and less discomfort than taking a sample with a swab deep into the nasal cavity or throat.
2) Because Medical workers do not need to collect samples separately, they can reduce their risk of disease infection. (In highly infected regions such as EU, the United States and many other countries, infection by medical personnel is a very serious problem.)
3) Saliva collection is faster and more scalable than swab collection, which can increase the number of people inspected each day.
1. Convenient and effective virus storage & Suitable for collecting SARS-CoV-2 samples.
2. Non-invasive approach for disease diagnosis and monitoring of general health.
3. Painless (no patient discomfort and anxiety for sampling).
4. Easy collection and applicable in remote areas & Suitable for on-site, home and clinic-based sampling).
5. Relatively cheap technology (Cheap to store and ship)
6. Cost-effective applicability for screening large populations.
7. Suitable for children, anxious/disabled/elderly patients.
8. Safer collection for health professionals than other biological samples such as nasopharyngeal swabs and blood.
9. Easy to handle & Possible multisampling.
10. No need for expensive equipment/instruments (swabs, suction tubes or special collection devices) for collection. Only needs a sterile container.
|
Symbol |
Explanation |
|---|---|
|
|
Catalog number |
|
|
CE mark |
|
|
In-vitro diagnostics medical device |
|
|
Batch code |
|
|
Expiration date |
|
|
Sufficient for <n> tests |
|
|
Consult instructions for use |
|
|
Temperature limit |
|
|
Do not re-use |
|
|
Do not re-sterilize |
|
|
Do not use if package is damaged. |
|
|
Caution, consult accompanying documents |
|
|
Manufacturer |
|
|
Manufacture date |
|
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
COVICHEK™ Foam Swabs are manufactured with over 150 pores per inch reticulated polyurethane foam, which ensures excellent absorbency while minimizing contamination.
Tips are affixed by heat seal to prevent tip loss, even while saturated with solvent.
Flexible tips provide versatility and softer touch.
COVICHEK™ Foam Swabs are manufactured with over 150 pores per inch reticulated polyurethane foam, which ensures excellent absorbency while minimizing contamination. Tips are affixed by heat seal to prevent tip loss, even while saturated with solvent. Flexible tips provide versatility and softer touch.
COVICHEK™ Spun Swabs made from closed-cell polyurethane foam with 100 pore per inch porosity, this swab possesses excellent durability and cushioning. And 100% virgin polypropylene handle ensures no additional contaminants are introduced while offering excellent chemical resistance.
|
Product image |
Swab Head |
Product name |
Length |
Breakpoint |
Use |
|---|---|---|---|---|---|
|
|
Foam_Oral Swab(Large) | 155mm | 30mm,84mm |
-. Specimen collection for DNA testing -. Optimized for COVID-19 sample collection -. Luminol test of minute blood stains |
|
|
Foam Oral Swab (Standard) | 152mm | 31mm | |
|
|
Foam Nasal Swab (Standard) | 152mm | 82mm | |
|
|
Spun Oral Swab (Standard) | 150mm | 26mm,80mm |
|
Product image |
Swab Head |
Product name |
|---|---|---|
|
|
Foam Oral Swab (Large) + Foam Nasal Swab (Standard) |
|
|
Foam Oral Swab (Standard) + Foam Nasal Swab (Standard) |
|
|
Spun Oral Swab (Standard) + Foam Nasal Swab (Standard) |
Simple and Fast | Wide Versatility | Safe | High Purity | High Yield
1) Simple and Fast : Extraction is completed in 30 minutes, and it can be used for automatic detection.
2) Wide Versatility : It is suitable for viral nucleic acid extraction from nasopharyngeal swab, saliva, sputum, bronchus lavage fluid and alveolar lavage fluid, etc.
3) Safe : No toxic reagents such as phenol and chloroform are needed in the extraction process.
4) High Purity : Remove inhibitive impurities to the maximum extent, the obtained high purity nucleic acid can be directly used for RT-qPCR.
5) High Yield : The nucleic acid recovery efficiency is very high, making the downstream more sensitive.
W 4001
The Kit consists of the following components.
|
No. |
Reagent |
Image |
Volume |
Description |
|---|---|---|---|---|
| 1 | Lysis Buffer |
|
30ml | Provide environment for lysing and binding to the column |
| 2 | Washing Buffer 1 |
|
80ml |
Remove residual proteins and other impurities (Add 60ml absolute ethanol before use) |
| 3 | Washing Buffer 2 |
|
80ml |
Purify viral nucleic acids (Add 72ml absolute ethanol before use) |
| 4 | Elution Buffer |
|
20ml | Nuclease-free solution, elute viral nucleic acids |
| 5 | Spin Column |
|
100pcs | Adsorb viral nucleic acid and collect the filtrate |
| 6 | 1.5ml Collection Tube |
|
100pcs | Collection of eluted viral nucleic acid |
Storage condition
Store at room temperature (15 ~ 25°C) and transport at room temperature.
Viral DNA/RNA Extraction
|
No. |
Step |
Image |
Description |
Note |
|---|---|---|---|---|
| 1 | Lysis |
|
Add lysis buffer to sample Sample: 200㎕ Lysis buffer: 500㎕ |
|
| 2 | Incubation |
|
1) Mix by vortexing for 15 seconds 2) Waiting for 10 min at room temperature |
|
| 3 | Precipitation |
|
1. Add absolute ethanol 400㎕ and mix. 2. Centrifuge and transfer supernant to spin column. |
12,000 x g for 1 minute |
| 4 | Column binding |
|
Centrifuge and discard the filtrate. | Centrifuge at 12,000ⅹg for 30~60 seconds |
| 5 | 1st Washing |
|
1) Add 700㎕ of washing buffer 1 to spin column. 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 6 | 2nd Washing |
|
1) Add 700㎕ of washing buffer 2 to spin column 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 7 | Elution |
|
1) Transfer the spin column to a new 1.5 ml Collection Tube (provided by the kit) 2) Add 50~100㎕ of elution buffer to spin column 3) Centrifuge |
Centrifuge at 13,000ⅹg for 3~5 minutes |
| 8 | Usage & Storage |
|
The obtained DNA/RNA can be directly used for the subsequent detection, or be store at -15 ~ -30℃ for short-term storage or at -70℃ long-term storage. |
Real-Time PCR Mix : One-Step Probe RT-qPCR KIT
RNA extracted from 2019 n-CoV abEN Pseudovirus by Swab Viral DNA/RNA Extraction Kit (Cat. No. W4001)
ORF1ab
N gene
|
Symbol |
Explanation |
|---|---|
|
|
Catalog number |
|
|
CE mark |
|
|
In-vitro diagnostics medical device |
|
|
Batch code |
|
|
Expiration date |
|
|
Sufficient for <n> tests |
|
|
Consult instructions for use |
|
|
Temperature limit |
|
|
Do not re-use |
|
|
Do not re-sterilize |
|
|
Do not use if package is damaged. |
|
|
Caution, consult accompanying documents |
|
|
Manufacturer |
|
|
Manufacture date |
|
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
1) Simple and Fast : Extraction is completed in 30 minutes, and it can be used for automatic detection.
2) Wide Versatility : It is suitable for viral nucleic acid extraction from nasopharyngeal swab, saliva, sputum, bronchus lavage fluid and alveolar lavage fluid, etc.
3) Safe : No toxic reagents such as phenol and chloroform are needed in the extraction process.
4) High Purity : Remove inhibitive impurities to the maximum extent, the obtained high purity nucleic acid can be directly used for RT-qPCR.
5) High Yield : The nucleic acid recovery efficiency is very high, making the downstream more sensitive.
W 4001
The Kit consists of the following components.
|
No. |
Reagent |
Image |
Volume |
Description |
|---|---|---|---|---|
| 1 | Lysis Buffer |
|
30ml | Provide environment for lysing and binding to the column |
| 2 | Washing Buffer 1 |
|
80ml |
Remove residual proteins and other impurities (Add 60ml absolute ethanol before use) |
| 3 | Washing Buffer 2 |
|
80ml |
Purify viral nucleic acids (Add 72ml absolute ethanol before use) |
| 4 | Elution Buffer |
|
20ml | Nuclease-free solution, elute viral nucleic acids |
| 5 | Spin Column |
|
100pcs | Adsorb viral nucleic acid and collect the filtrate |
| 6 | 1.5ml Collection Tube |
|
100pcs | Collection of eluted viral nucleic acid |
Storage condition
Store at room temperature (15 ~ 25°C) and transport at room temperature.
Viral DNA/RNA Extraction
|
No. |
Step |
Image |
Description |
Note |
|---|---|---|---|---|
| 1 | Lysis |
|
Add lysis buffer to sample Sample: 200㎕ Lysis buffer: 500㎕ |
|
| 2 | Incubation |
|
1) Mix by vortexing for 15 seconds 2) Waiting for 10 min at room temperature |
|
| 3 | Precipitation |
|
1. Add absolute ethanol 400㎕ and mix. 2. Centrifuge and transfer supernant to spin column. |
12,000 x g for 1 minute |
| 4 | Column binding |
|
Centrifuge and discard the filtrate. | Centrifuge at 12,000ⅹg for 30~60 seconds |
| 5 | 1st Washing |
|
1) Add 700㎕ of washing buffer 1 to spin column. 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 6 | 2nd Washing |
|
1) Add 700㎕ of washing buffer 2 to spin column 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 7 | Elution |
|
1) Transfer the spin column to a new 1.5 ml Collection Tube (provided by the kit) 2) Add 50~100㎕ of elution buffer to spin column 3) Centrifuge |
Centrifuge at 13,000ⅹg for 3~5 minutes |
| 8 | Usage & Storage |
|
The obtained DNA/RNA can be directly used for the subsequent detection, or be store at -15 ~ -30℃ for short-term storage or at -70℃ long-term storage. |
Real-Time PCR Mix : One-Step Probe RT-qPCR KIT
RNA extracted from 2019 n-CoV abEN Pseudovirus by Swab Viral DNA/RNA Extraction Kit (Cat. No. W4001)
ORF1ab
N gene
|
Symbol |
Explanation |
|---|---|
|
|
Catalog number |
|
|
CE mark |
|
|
In-vitro diagnostics medical device |
|
|
Batch code |
|
|
Expiration date |
|
|
Sufficient for <n> tests |
|
|
Consult instructions for use |
|
|
Temperature limit |
|
|
Do not re-use |
|
|
Do not re-sterilize |
|
|
Do not use if package is damaged. |
|
|
Caution, consult accompanying documents |
|
|
Manufacturer |
|
|
Manufacture date |
|
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
1) Simple and Fast : Extraction is completed in 30 minutes, and it can be used for automatic detection.
2) Wide Versatility : It is suitable for viral nucleic acid extraction from nasopharyngeal swab, saliva, sputum, bronchus lavage fluid and alveolar lavage fluid, etc.
3) Safe : No toxic reagents such as phenol and chloroform are needed in the extraction process.
4) High Purity : Remove inhibitive impurities to the maximum extent, the obtained high purity nucleic acid can be directly used for RT-qPCR.
5) High Yield : The nucleic acid recovery efficiency is very high, making the downstream more sensitive.
W 4001
The Kit consists of the following components.
|
No. |
Reagent |
Image |
Volume |
Description |
|---|---|---|---|---|
| 1 | Lysis Buffer |
|
30ml | Provide environment for lysing and binding to the column |
| 2 | Washing Buffer 1 |
|
80ml |
Remove residual proteins and other impurities (Add 60ml absolute ethanol before use) |
| 3 | Washing Buffer 2 |
|
80ml |
Purify viral nucleic acids (Add 72ml absolute ethanol before use) |
| 4 | Elution Buffer |
|
20ml | Nuclease-free solution, elute viral nucleic acids |
| 5 | Spin Column |
|
100pcs | Adsorb viral nucleic acid and collect the filtrate |
| 6 | 1.5ml Collection Tube |
|
100pcs | Collection of eluted viral nucleic acid |
Storage condition
Store at room temperature (15 ~ 25°C) and transport at room temperature.
Viral DNA/RNA Extraction
|
No. |
Step |
Image |
Description |
Note |
|---|---|---|---|---|
| 1 | Lysis |
|
Add lysis buffer to sample Sample: 200㎕ Lysis buffer: 500㎕ |
|
| 2 | Incubation |
|
1) Mix by vortexing for 15 seconds 2) Waiting for 10 min at room temperature |
|
| 3 | Precipitation |
|
1. Add absolute ethanol 400㎕ and mix. 2. Centrifuge and transfer supernant to spin column. |
12,000 x g for 1 minute |
| 4 | Column binding |
|
Centrifuge and discard the filtrate. | Centrifuge at 12,000ⅹg for 30~60 seconds |
| 5 | 1st Washing |
|
1) Add 700㎕ of washing buffer 1 to spin column. 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 6 | 2nd Washing |
|
1) Add 700㎕ of washing buffer 2 to spin column 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 7 | Elution |
|
1) Transfer the spin column to a new 1.5 ml Collection Tube (provided by the kit) 2) Add 50~100㎕ of elution buffer to spin column 3) Centrifuge |
Centrifuge at 13,000ⅹg for 3~5 minutes |
| 8 | Usage & Storage |
|
The obtained DNA/RNA can be directly used for the subsequent detection, or be store at -15 ~ -30℃ for short-term storage or at -70℃ long-term storage. |
Real-Time PCR Mix : One-Step Probe RT-qPCR KIT
RNA extracted from 2019 n-CoV abEN Pseudovirus by Swab Viral DNA/RNA Extraction Kit (Cat. No. W4001)
ORF1ab
N gene
|
Symbol |
Explanation |
|---|---|
|
|
Catalog number |
|
|
CE mark |
|
|
In-vitro diagnostics medical device |
|
|
Batch code |
|
|
Expiration date |
|
|
Sufficient for <n> tests |
|
|
Consult instructions for use |
|
|
Temperature limit |
|
|
Do not re-use |
|
|
Do not re-sterilize |
|
|
Do not use if package is damaged. |
|
|
Caution, consult accompanying documents |
|
|
Manufacturer |
|
|
Manufacture date |
|
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
1) Simple and Fast : Extraction is completed in 30 minutes, and it can be used for automatic detection.
2) Wide Versatility : It is suitable for viral nucleic acid extraction from nasopharyngeal swab, saliva, sputum, bronchus lavage fluid and alveolar lavage fluid, etc.
3) Safe : No toxic reagents such as phenol and chloroform are needed in the extraction process.
4) High Purity : Remove inhibitive impurities to the maximum extent, the obtained high purity nucleic acid can be directly used for RT-qPCR.
5) High Yield : The nucleic acid recovery efficiency is very high, making the downstream more sensitive.
W 4001
The Kit consists of the following components.
|
No. |
Reagent |
Image |
Volume |
Description |
|---|---|---|---|---|
| 1 | Lysis Buffer |
|
30ml | Provide environment for lysing and binding to the column |
| 2 | Washing Buffer 1 |
|
80ml |
Remove residual proteins and other impurities (Add 60ml absolute ethanol before use) |
| 3 | Washing Buffer 2 |
|
80ml |
Purify viral nucleic acids (Add 72ml absolute ethanol before use) |
| 4 | Elution Buffer |
|
20ml | Nuclease-free solution, elute viral nucleic acids |
| 5 | Spin Column |
|
100pcs | Adsorb viral nucleic acid and collect the filtrate |
| 6 | 1.5ml Collection Tube |
|
100pcs | Collection of eluted viral nucleic acid |
Storage condition
Store at room temperature (15 ~ 25°C) and transport at room temperature.
Viral DNA/RNA Extraction
|
No. |
Step |
Image |
Description |
Note |
|---|---|---|---|---|
| 1 | Lysis |
|
Add lysis buffer to sample Sample: 200㎕ Lysis buffer: 500㎕ |
|
| 2 | Incubation |
|
1) Mix by vortexing for 15 seconds 2) Waiting for 10 min at room temperature |
|
| 3 | Precipitation |
|
1. Add absolute ethanol 400㎕ and mix. 2. Centrifuge and transfer supernant to spin column. |
12,000 x g for 1 minute |
| 4 | Column binding |
|
Centrifuge and discard the filtrate. | Centrifuge at 12,000ⅹg for 30~60 seconds |
| 5 | 1st Washing |
|
1) Add 700㎕ of washing buffer 1 to spin column. 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 6 | 2nd Washing |
|
1) Add 700㎕ of washing buffer 2 to spin column 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 7 | Elution |
|
1) Transfer the spin column to a new 1.5 ml Collection Tube (provided by the kit) 2) Add 50~100㎕ of elution buffer to spin column 3) Centrifuge |
Centrifuge at 13,000ⅹg for 3~5 minutes |
| 8 | Usage & Storage |
|
The obtained DNA/RNA can be directly used for the subsequent detection, or be store at -15 ~ -30℃ for short-term storage or at -70℃ long-term storage. |
Real-Time PCR Mix : One-Step Probe RT-qPCR KIT
RNA extracted from 2019 n-CoV abEN Pseudovirus by Swab Viral DNA/RNA Extraction Kit (Cat. No. W4001)
ORF1ab
N gene
|
Symbol |
Explanation |
|---|---|
|
|
Catalog number |
|
|
CE mark |
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In-vitro diagnostics medical device |
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Batch code |
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Expiration date |
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Sufficient for <n> tests |
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Consult instructions for use |
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Temperature limit |
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Do not re-use |
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Do not re-sterilize |
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Do not use if package is damaged. |
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Caution, consult accompanying documents |
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Manufacturer |
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Manufacture date |
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Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
1) Simple and Fast : Extraction is completed in 30 minutes, and it can be used for automatic detection.
2) Wide Versatility : It is suitable for viral nucleic acid extraction from nasopharyngeal swab, saliva, sputum, bronchus lavage fluid and alveolar lavage fluid, etc.
3) Safe : No toxic reagents such as phenol and chloroform are needed in the extraction process.
4) High Purity : Remove inhibitive impurities to the maximum extent, the obtained high purity nucleic acid can be directly used for RT-qPCR.
5) High Yield : The nucleic acid recovery efficiency is very high, making the downstream more sensitive.
W 4001
The Kit consists of the following components.
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No. |
Reagent |
Image |
Volume |
Description |
|---|---|---|---|---|
| 1 | Lysis Buffer |
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30ml | Provide environment for lysing and binding to the column |
| 2 | Washing Buffer 1 |
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80ml |
Remove residual proteins and other impurities (Add 60ml absolute ethanol before use) |
| 3 | Washing Buffer 2 |
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80ml |
Purify viral nucleic acids (Add 72ml absolute ethanol before use) |
| 4 | Elution Buffer |
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20ml | Nuclease-free solution, elute viral nucleic acids |
| 5 | Spin Column |
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100pcs | Adsorb viral nucleic acid and collect the filtrate |
| 6 | 1.5ml Collection Tube |
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100pcs | Collection of eluted viral nucleic acid |
Storage condition
Store at room temperature (15 ~ 25°C) and transport at room temperature.
Viral DNA/RNA Extraction
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No. |
Step |
Image |
Description |
Note |
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| 1 | Lysis |
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Add lysis buffer to sample Sample: 200㎕ Lysis buffer: 500㎕ |
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| 2 | Incubation |
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1) Mix by vortexing for 15 seconds 2) Waiting for 10 min at room temperature |
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| 3 | Precipitation |
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1. Add absolute ethanol 400㎕ and mix. 2. Centrifuge and transfer supernant to spin column. |
12,000 x g for 1 minute |
| 4 | Column binding |
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Centrifuge and discard the filtrate. | Centrifuge at 12,000ⅹg for 30~60 seconds |
| 5 | 1st Washing |
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1) Add 700㎕ of washing buffer 1 to spin column. 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 6 | 2nd Washing |
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1) Add 700㎕ of washing buffer 2 to spin column 2) Centrifuge 3) Discard the filtrate |
Centrifuge at 12,000ⅹg for 30~60 seconds |
| 7 | Elution |
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1) Transfer the spin column to a new 1.5 ml Collection Tube (provided by the kit) 2) Add 50~100㎕ of elution buffer to spin column 3) Centrifuge |
Centrifuge at 13,000ⅹg for 3~5 minutes |
| 8 | Usage & Storage |
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The obtained DNA/RNA can be directly used for the subsequent detection, or be store at -15 ~ -30℃ for short-term storage or at -70℃ long-term storage. |
Real-Time PCR Mix : One-Step Probe RT-qPCR KIT
RNA extracted from 2019 n-CoV abEN Pseudovirus by Swab Viral DNA/RNA Extraction Kit (Cat. No. W4001)
ORF1ab
N gene
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Symbol |
Explanation |
|---|---|
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Catalog number |
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CE mark |
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In-vitro diagnostics medical device |
|
|
Batch code |
|
|
Expiration date |
|
|
Sufficient for <n> tests |
|
|
Consult instructions for use |
|
|
Temperature limit |
|
|
Do not re-use |
|
|
Do not re-sterilize |
|
|
Do not use if package is damaged. |
|
|
Caution, consult accompanying documents |
|
|
Manufacturer |
|
|
Manufacture date |
|
|
Authorized representative in the European Community |
Manufacturer
Bio Ventrue Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea
In order to manufacture safe and high-quality products, we have secured FDA, CE, GMP facilities and manufacture them with permission from the Korean FDA based on ISO 13485, prepare all kinds of documentary evidencesuch as E.O gas for sterilization, Certificate of Analysis, Gamma- Irradiation, CE mark certification,
ISO, etc. and sell them in Korea and abroad.
For further information, please contact WIZCHEM.
Bio Venture Town 401-404, Daejeon Techno-Park, 1662, Yuseong-daero, Yuseong-gu, Daejeon, 34054, Korea
112, Oksansandan 4-ro, Oksan-myeon, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 28111, Korea
104, Munpyeongdong-ro 48beon-gil, Daedeok-gu, Daejeon, Korea
Development of functional chemicals and electronic chemical materials
Bio-medi cosmetics with technologies derived from various skin physiological substances
For first aid, hydrofluoric acid burn skin external preparation, hydrofluoric acid neutralizer, etc.
Break Point